A test that is performed on blood (after centrifugation to remove red blood cells from the blood) to detect AIDS antibodies individually. Gel electrophoresis is used to separate the AIDS antigen proteins of killed (known) AIDS viruses. Next the protein bands (resulting from the gel electrophoresis) are exposed to the blood being tested and (AIDS) antibodies stick to specific individual antigens (bands) which are then identified (as being present in the tested blood) via dyes.
A laboratory blood test to identify and analyze specific protein antigens; sometimes used to confirm the validity of the ELISA test.
A technique for analyzing protein antigens. Initially, the antigens are separated by electrophoresis on a gel and transferred to a solid membrane by blotting. The membrane is incubated with antibodies, and then the bound antibodies are detected by enzymatic or radioactive methods. This method is used to detect small amounts of antibodies.