Optical density (CD)

The absorbance of light of a specific wavelength by molecules normally dissolved in a solution. Light absorption depends upon the concentration of the absorbing compound (chemical entity) in the solution, the thickness of the sample being illuminated, and the chemical nature of the absorbing compound. An analytical instrument known as a spectrophotometer is used to (quantitatively) express the amount of a substance (dissolved) in a solution. Mathematically, this is accomplished using the Beer-Lambert Law.


A number describing the blackening of an x-ray film in any specified location. In general the optical density is the log of the ratio of incident to transmitted light through that area and is measured with a densitometer.


The ability of a laboratory specimen to absorb or block the passage of light. The optical density of a laboratory sample can be used as an indicator of the concentration of specific components in the sample.


 


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