Northern blotting

Widely used technique for detecting mRNAs by hybridisation with specific probes following transfer of RNA onto a solid support, such as a nylon membrane; see mRNA, RNA, hybridisation.


A research test/methodology used to transfer RNA fragments from an agarose gel (e.g., following gel electrophoresis) to a filter paper without changing the relative positions of the RNA fragments (e.g., re electrophoresis separation grid).


A method for transferring RNA from an agarose gel to a nitrocellulose filter on which the RNA can be detected using a suitable probe.


 


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